Specimens collected at autopsy are often contaminated with faecal or skin flora making the interpretation of cultures very difficult. The finding of a specific organism may be very noteworthy, however.
Prepare 2 touch preparations using a freshly cut surface of representative material. Gram stain one and retain the other for potential future stains.
Macerate the tissue using a grinder. Bone should be inoculated into Thioglycolate broth and not macerated.
|Blood Agar (BA)||CO2, 35°C x 48 hours|
|Chocolate Agar (CHOC)||CO2, 35°C x 48 hours|
|MacConkey Agar (MAC)||O2, 35°C x 48 hours|
|Phenyl Ethyl Alcohol Agar (PEA)||O2, 35°C x 48 hours|
For all lung specimens or if fungal culture specifically requested:
|Sabouraud’s Heart Infusion Agar (SABHI)||O2, 30°C x 4 weeks|
|SABHI with Chloramphenicol (SABHI-C)||O2, 30°C x 4 weeks|
Report with quantitation the presence of pus cells and organisms.
Negative Report “No growth” or “Mixed flora suggesting contamination”
Positive Report Report all significant isolates WITHOUT susceptibilities.
H.D. Isenberg, 2004. Wound Cultures – Wound and Soft Tissue Cultures, p. 184.108.40.206 – 220.127.116.11. In Clinical Microbiology Procedures Handbook, 2nd Edition, Vol 1 ASM Press, Washington, D.C.