Bile is a normally sterile fluid. The preferred sample is obtained at the time of initial drainage which is often done in the radiology department.
Samples are often sent from drains that have been left in the biliary tract. The finding of microorganisms in samples from indwelling drains is much more difficult to interpret as the drains are commonly colonized with skin flora.
|Blood Agar (BA)||CO2, 35°C x 48 hours|
|MacConkey Agar (MAC)||O2, 35°C x 48 hours|
If sample was collected in radiology or the operating room or if anaerobic culture is specifically requested, add:
|Wilkins Chalgren Agar (WC)||AnO2, 35°C x 48 hours|
|WC Nalidixic acid – Tween 80 (NAT)||AnO2, 35°C x 48 hours|
|WC Nalidixic acid – Vancomycin (NAV)||AnO2, 35°C x 48 hours|
|Thioglycolate broth (THIO)||O2, 35°C x 48 hours|
For other organisms, a significant result is determined by the isolation of <2 organisms. Consult with charge technologist or microbiologist if unsure.
All non-lactose fermenters (NLF) should be screened for Salmonella spp.
Report without quantitation the presence of pus cells and organisms.
Negative Report “No growth” or “Mixed faecal flora”
Positive Report Report all significant isolates with appropriate susceptibilities, without quantitation. If faecal flora is also present, report without quantitation
H.D. Isenberg, 2004. Wound Cultures – Wound and Soft Tissue Cultures, p. 126.96.36.199 – 188.8.131.52. In Clinical Microbiology Procedures Handbook, 2nd Edition, Vol 1 ASM Press, Washington, D.C.
H.D. Isenberg. 2004. Culture for anaerobes p. 4.3.1 – 4.3.9 In Clinical Microbiology Procedures Handbook, 2nd Edition, Vol 1 ASM Press, Washington, D.C.