Breast milk

Breast milk may become infected with a variety of organisms and all species should be identified except normal skin flora.

Setup

Direct Examination

Not required

Culture

Media Incubation
Blood Agar (BA) CO2, 35°C x 48 hours
MacConkey Agar (MAC) O2, 35°C x 48 hours

Interpretation Examine the culture plates after 24 and 48 incubation. Any growth of organisms other than normal skin flora should be considered significant.
Reporting

Negative Report

“No growth” or “Normal skin flora”

Positive Report

Quantitate all significant isolates with appropriate sensitivities. If normal skin flora is also present, report with quantitation.

References H.D. Isenberg. 2004. Specimen Collection, Transport and Acceptability p. 2.1.1 – 2.1.28. In Clinical Microbiology Procedures handbook, 2nd Edition, Vol 1 ASM Press, Washington, D.C

H.D. Isenberg, 2004. Wound Cultures – Wound and Soft Tissue Cultures, p. 3.13.1.1 – 3.13.1.16. In Clinical Microbiology Procedures Handbook, 2nd Edition, Vol 1 ASM Press, Washington, D.C.