Occasionally blood, platelets and other transfusion products become contaminated at the time of collection from donors, during processing or at the time of infusion into patients. Any organism isolated must be considered significant.
From the bag aspirate 20 mL and inoculate aerobic and anaerobic blood culture bottle with approximately 9 mL each. Process as per usual blood culture protocol.
With the remaining blood aspirated from the bag and with the sample retrieved from the segment inoculate:
|Blood Agar (BA)||CO2, 35°C x 4 days|
|Chocolate Agar (CHOC)||CO2, 35°C x 4 days|
Examine the BA and CHOC plates for growth daily for 4 days.
Report all isolates with appropriate susceptibilities.
Do not quantitate except if it is from the fluid medium only – add Comment “From broth culture only, indicative of small numbers and/or contamination.”
Telephone results of all positive Gram stains and cultures to ward / ordering physician.
H.D. Izenberg. 2003. Blood Cultures-General Detection and Interpretation, p.126.96.36.199-188.8.131.52 In Clinical Microbiology Procedures Handbook, 2nd ed. Vol.1 ASM Press, Washington, D.C.