The recognized agents of cervicitis are Neisseria gonorrhoeae (GC), Chlamydia trachomatis (CT) and Herpes simplex virus (HSV). CT and HSV require specific samples and processing.
In contrast to samples from the urethra, Gram stain is not reliable for the presumptive diagnosis of GC cervicitis because the potential presence of commensal Neisseria spp. limits the specificity. It is not performed.
|Vancomycin-Colistin-Nystatin Agar (VCN)||CO2, 35°C x 72 hours|
No Neisseria gonorrhoeae isolated.
If VCN plate is overgrown by swarming Proteus or yeast, report ONLY as “Unable to rule out Neisseria gonorrhoeae due to bacterial/yeast overgrowth.”
Insert “Neisseria gonorrhoeae” isolated (do not quantitate), beta lactamase non-producing or producing strain. Insert comment “Report has been copied to Medical Officer of Health”.
Telephone all positive GC cultures to floor/ordering physician.
For all positive GC cultures, a Communicable Disease Report is sent to the Medical Officer of Health and is recorded in the significant isolates log book.
Cumitech 17A, 1993. Laboratory Diagnosis of Female Genital Tract Infectious, ASM Press.
Survey B-9412, Feb. 21, 1995. Microbiology Handling of Female Genital Specimens. A pattern of Practice Survey.