Although any organism may cause infection of the placenta, the most common organisms associated with this syndrome include S. aureus, beta hemolytic streptococci, Listeria monocytogenes and E. coli.
Neisseria gonorrhoeae is a possibility and occasionally Mycobacterium tuberculosis will be considered and requested.
Prepare 2 smears: one for Gram stain and one to be held in reserve.
|Blood Agar (BA)||CO2, 35°C x 48 hours|
|Chocolate Agar (CHOC)||CO2, 35°C x 48 hours|
|Vancomycin-Colistin-Nystatin Agar (VCN)||CO2, 35°C x 72 hours|
|MacConkey Agar (MAC)||O2, 35°C x 48 hours|
Report with quantitation the presence of pus cells and organisms.
“No significant growth” or “ No growth” and “No Neisseria gonorrhoeae isolated”.
“Neisseria gonorrhoeae” isolated (do not quantitate), beta lactamase negative or positive. “Report has been copied to Medical Officer of Health”.
Quantitate and report all other significant isolates with appropriate susceptibility results.
Telephone all positive GC cultures to floor/ordering Physician.
For all positive GC cultures, a Communicable Disease Report is sent to the Medical Officer of Health and recorded in the significant isolates log book.
Bailey & Scott’s Diagnostic Microbiology. Finegold & Baron; 7th. Ed., C.V. Mosby Co. p. 301.