A variety of organisms may be associated with skin lesions and thus any growth of organisms other than normal skin flora should be considered significant.
Macerate the entire (or remaining) specimen with a small volume of sterile saline from the original container using a grinder.
If fungus is requested, add: KOH Calcofluor stain
|Blood Agar (BA)||CO2, 35°C x 48 hours|
|Chocolate Agar (CHOC)||CO2, 35°C x 48 hours|
|MacConkey Agar (MAC)||O2, 35°C x 48 hours|
If fungus requested, add:
|Sabouraud’s Heart Infusion Agar (SABHI)||O2, 30°C x 4 weeks|
|SABHI with Chloramphenicol (SABHI-C)||O2, 30°C x 4 weeks|
Report with quantitation the presence of pus cells and organisms.
“No growth” or “Normal skin flora”
Quantitate all significant isolates with appropriate susceptibilities. If other organisms are also present, report as “Normal skin flora” with quantitation.
H.D. Isenberg, 2004. Wound Cultures – Wound and Soft Tissue Cultures, p. 126.96.36.199 – 188.8.131.52. In Clinical Microbiology Procedures Handbook, 2nd Edition, Vol 1 ASM Press, Washington, D.C.
Cumitech 23 Infections of the Skin and Subcutaneous Tissues June 1988